Improved method for estimating blood sugar.

نویسندگان

  • N C Sharma
  • B K Sur
چکیده

Received for publication 8 December 1965. reduced copper is oxidized to cupric ions by means of iodine. If no necessary allowance has been made, the cupric ions formed would be completely reduced and precipitated as cuprous iodide in the presence of an excess of soluble iodide in the reagent. Probably to check this reduction by iodide, Harding and Downs (1933) employed potassium oxalate in their alkaline reagent because oxalate inhibits more or less completely the reaction of the cupric salt with soluble iodides by forming alkali cuprioxalate which dissociates to give cuprioxalate anions (Elbs, 1917; Shaffer and Hartmann, 1921; DeLong, 1927). Asatoor and King (1954), without considering the fundamental role of potassium oxalate, retained it in the alkaline reagent in their colorimetric modification of the method which makes no use of iodometry. It appears that potassium oxalate plays no role in the colorimetric method and, therefore, we modified the methodofA satoor and King by omitting it in their alkaline reagent.

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عنوان ژورنال:
  • Journal of clinical pathology

دوره 19 6  شماره 

صفحات  -

تاریخ انتشار 1966